Forskning

November 9, 2020

A new UPLC-qTOF approach for elucidating furan and 2-methylfuran metabolites in human urine samples after coffee consumption

S Stegmuller et al, 2020. A new UPLC-qTOF approach for elucidating furan and 2-methylfuran metabolites in human urine samples after coffee consumption, Molecules, Volume 25 (21).

ABSTRACT:

We have investigated urine samples after coffee consumption using targeted and untargeted approaches to identify furan and 2-methylfuran metabolites in urine samples by UPLC-qToF. The aim was to establish a fast, robust, and time-saving method involving ultra-performance liquid chromatography-quantitative time-of-flight tandem mass spectrometry (UPLC-qToF-MS/MS). The developed method detected previously reported metabolites, such as Lys-BDA, and others that had not been previously identified, or only detected in animal or in vitro studies. The developed UPLC-qToF method detected previously reported metabolites, such as lysine-cis-2-butene-1,4-dial (Lys-BDA) adducts, and others that had not been previously identified, or only detected in animal and in vitro studies. In sum, the UPLC-qToF approach provides additional information that may be valuable in future human or animal intervention studies.