Forskning

October 26, 2016

Evaluation of the antioxidant capacity, furan compounds and cytoprotective/cytotoxic effects upon Caco-2 cells of commercial Colombian coffee

B Bedoya-Ramirez et al, 2017. Evaluation of the antioxidant capacity, furan compounds and cytoprotective/cytotoxic effects upon Caco-2 cells of commercial Colombian coffee, Food Chemistry, published online.

ABSTRACT:

Antioxidant capacity (AC), total phenolics (TPs), furan compounds (HMF and furfural F) and cytoprotective/cytotoxic effects upon Caco-2 cells (MTT, cell cycle and reactive oxygen species (ROS)) were evaluated in Colombian coffee (2 ground and 4 soluble samples). The AC (ABTS and FRAP), TPs and HMF ranged between 124–722, 95–802 lmoles Trolox/g, 21–100 mg gallic acid/g and 69–2900 mg/kg, respectively. Pretreatment of cells for 24 h with lyophilized coffee infusions at the highest dose without cytotoxic effects (500 lg/mL) significantly prevented the decrease in cell viability compared to control stress with H2O2 (5 mM/2 h), recovering viability to values between 34% and 45% and restoring the control values without stress induction in the G1 phase of cell cycle. After exposure to stress, four extracts decreased ROS values significantly to 22.5–24.9%. The coffee samples exerted a cytoprotective effect against oxidative stress, with improvement in cell viability and a reduction of intracellular ROS