January 24, 2014
A Riedel et al, 2014, N-Methylpyridinium, a degradation product of trigonelline upon coffee roasting, stimulates respiratory activity and promotes glucose utilization in HepG2 cells, Food and Function, published online ahead of print.
N-Methylpyridinium (NMP) is a thermal degradation product of trigonelline formed upon coﬀee roasting and hypothesized to exert several health beneﬁts in humans. Since for trigonelline evidence for hypoglycemic eﬀects exists, we examined whether NMP also aﬀects mechanisms of glucose utilization and cellular energy formation. For this purpose, the impact of trigonelline and NMP on respiratory activity, extracellular acidiﬁcation, cellular adenosine nucleotides, energy supply from fatty acids and glucose as well as thermogenesis in HepG2 cells was analyzed. A 24 hour incubation with nanomolar concentrations of NMP enhanced oxygen consumption rates, resulting in increased ATP levels. Glucose was identiﬁed as the prevalent energy substrate as its uptake was augmented up to 18.1% 7.44% by NMP at 0.09 mM, whereas the uptake of fatty acids decreased upon NMP treatment. Cellular glucose uptake was also stimulated by trigonelline administration; however, a shift to the anaerobic energy production pathway was monitored. Both pyridine derivatives induced thermogenesis, although trigonelline presumably promoted proton leaks, while NMP increased the concentration of the uncoupling protein-2. We provide evidence that both compounds appear to stimulate cellular energy metabolism in HepG2 cells. Human intervention studies are warranted to ensure these eﬀects in vivo
Ja tak, jeg vil gerne modtage nyhedsbrev, når der er noget nyt om kaffe og helbred.