Forskning

June 13, 2018

Javamide-II found in coffee is better than caffeine in suppressing TNF-alpha production in PMA/PHA-treated lymphocytic Jurkat cells

J B Park, 2018. Javamide-II found in coffee is better than caffeine in suppressing TNF-alpha production in PMA/PHA-treated lymphocytic Jurkat cells, Journal of Agric and Food Chem, published online.

ABSTRACT:

Recent studies suggested positive benefits of coffee consumption on inflammation-related diseases such as liver diseases and diabetes where activated lymphocytes and TNF-alpha are critically implicated. Interestingly, some reports suggested that javamide-II found in coffeemay have anti-inflammation activity greater than caffeine, but there is limited information about its effect on TNF-alpha production by activated lymphocytes. Therefore, the inhibitory effect of javamide-II on TNF-alpha was investigated in PMA/PHA-treated lymphocytic Jurkat cells. At 5 µM, javamide-II, not caffeine, inhibited TNF-alpha production in the cells (45±4 %; P < 0.001). To elucidate the underlying mechanism, the phosphorylation of MAP kinases (ERK, p38, JNK) was investigated in the Jurkat cells. Javamide-II had little effect on JNK or p38 phosphorylation, but javamide-II (< 20 µM) decreased ERK phosphorylation, consequently reducing TNF-alpha mRNA expression in the cells (P < 0.001). The involvement of ERK phosphorylation was also confirmed by a ERK1/2 inhibitor (SCH772984). Furthermore, javamide-II was also found to inhibit IL-2 production up-regulated by ERK phosphorylation in the cells (P < 0.001). These data suggested that javamide-II may be a potent compound to suppress TNF-alpha production more efficiently than caffeine by inhibiting ERK phosphorylation in the Jurkat cells.