Forskning

January 5, 2015

Development of a sensitive method for the determination of acrylamide in coffee using high performance liquid chromatography coupled to a hybrid quadrupole orbitrap mass sprectrometer

I Pugajeva et al, 2014, Development of a sensitive method for the determination of acrylamide in coffee using high performance liquid chromatography coupled to a hybrid quadrupole orbitrap mass sprectrometer, Food Additives and Contaminants: Part A, published online ahead of print.

ABSTRACT: The emerging trend towards high-resolution mass spectrometry alternatives was evaluated by the application of Orbitrap mass spectrometry for the determination of acrylamide in coffee samples. The high resolving power of the Orbitrap mass spectrometer provided the high selectivity and sensitivity that enabled quantitative analysis of acrylamide in complex matrices, such as coffee. Several sample preparation methods and scanning modes of the mass spectrometer (full MS, t-SIM, t-MS2) were assessed in order to optimise parameters of the analytical method. The final procedure involved the extraction of acrylamide with acetonitrile, solid phase extraction with dispersive PSA and amino columns, and the detection by ultra-performance liquid chromatography coupled to a hybrid quadrupole-Orbitrap mass spectrometer (HPLC-Q-Orbitrap) operated in targeted MS2 scanning mode. The repeatability of the method at the lowest calibration level (10 μg kg-1), expressed as relative standard deviation, was 7.8% and the average recovery of acrylamide was 111%. The proposed method was applied to the determination of acrylamide in 22 samples of roasted coffee obtained from the Latvian retail market. Acrylamide concentration in coffee samples was in the range of 166-503 μg kg-1.