September 15, 2011
T Sauer et al, Nuclear translocation of NF-kB in intact human gut tissue upon stimulation with coffee and roasting products, Food and Function, 2011.
ABSTRACT: In the healthy gut, NF-kB is a critical factor of the intestinal immune system, whereas inflammatory bowel diseases are associated with chronic activation of NF-kB. Previous studies indicated that coffee induces nuclear translocation of NF-kB in macrophages, an effect attributed to roasting products. In the present work, coffee extract or roasting products induced nuclear translocation of NF-kB in macrophages, Caco-2 cells, and primary human intestinal microvascular endothelial cells (up to fivefold, p < 0.001). Since the effect clearly depended on the cell type, ex vivo experiments were performed with intact human gut tissue from biopsies. The uniformity of the specimens and tissue viability during ex vivo incubation for up to 2 h were verified. Roasting products led to a concentration dependent significant increase of nuclear translocation of NF-kB in human gut tissue (up to 2.85 fold increase, p = 0.0321), whereas coffee extract induced a trend towards higher nuclear NF-kB concentration. NF-kB activation in macrophages and Caco-2 cells by roasting products was significantly blocked by co-incubation with catalase (p=0.011 and p=0.024) indicating involvement ofH2O2-signaling. Monitoring of extracellular H2O2 indicated that roasting products in coffee constantly generate H2O2 by spontaneous oxygen reduction, which is only partially detoxified by cellular antioxidative systems. Thus, it can be concluded that ex vivo stimulation of intact human gut tissue is a valuable model to study nutritional effects on complex tissue systems. Furthermore, the consumption of coffee and roasting products may be able to induce nuclear NF-kB translocation in the human gut.
Ja tak, jeg vil gerne modtage nyhedsbrev, når der er noget nyt om kaffe og helbred.